Fig. 8

Complementarity determining region heavy 3 (CDRH3) signatures in acute DENV infection. A search for convergent CDRH3 signatures was performed using previously published data [41] or de novo, using the Find_CDR3 function of ImmunediveRsity [30]. a Proportion of identical or similar (one-mismatch) CDRH3s in our acute and post-convalescent datasets compared to a set of 151 convergent CDRH3s associated with acute DENV infection previously described [41]. b CDRH3 length distribution of de novo identified convergent CDRH3 signatures compared to overall CDRH3 length distribution. We found 1365 clonotypes representing 269 identical CDRH3 shared in at least three individuals with acute DENV infection. A predominant 10-residue CDRH3 signature was found. c Amino acid residue composition in 10-residue-long CDRH3 (left) and in 10-residue-long de novo identified convergent CDRH3s associated with DENV infection (right). d LOGO plot of consensus 10-residue-long convergent CDRH3 signatures. e Absolute number of shared CDRH3s as a function of the number of individuals who share them. f Proportion of somatic hypermutation per lineage in convergent and non-convergent CDRH3s in acute DWS – (green) and DWS+ (blue). DWS − dengue without warning signs; DWS + dengue with warning signs