Fig. 1

The IL-4 regulated microRNome in human alternatively activated macrophages. a Two-dimensional principle component analysis of the miRNA transcriptome of human peripheral blood-derived monocytes, as well as unstimulated and IL-4-stimulated differentiated macrophages obtained from three independent donors. Principal component analysis was carried out on all miRNAs expressed in monocytes and/or macrophages (500 miRNAs). Color scheme: monocytes (Mo.), red; 12-h nontreated (nt.) differentiating macrophages, blue; 12-h IL-4 stimulated differentiating macrophages, light green; 72-h nontreated macrophages, dark green; and 72-h IL-4-stimulated macrophages, orange. b Heatmap showing average fold changes and significance of the top ten IL-4 upregulated and downregulated miRNAs (FDR ˂0.1) in 72-h unstimulated macrophages compared with monocytes (left panel) and 72-h IL-4-treated macrophages compared with 72-h unstimulated macrophages (right panel) from three independent human donors. c Stem-loop RT-qPCR-based measurement of miR-342-3p, miR-193b, miR-99b, and miR-125a-5p expression in human monocytes, 72-h nontreated, and IL-4-stimulated macrophages. Bars show the mean ± standard deviation of normalized signal intensity values from four independent human donor-derived cells. *P ˂ 0.05, **P ˂ 0.01, ***P ˂ 0.001; n.s. not significant