Fig. 7

Reduced cell viability and increased macrophage apoptosis by miR-342-3p overexpression. a PI staining-based cell number analysis of RAW264. Seven cells at different time points following miR-342-3p mimic transfection. Each data point represents the mean and standard error of the mean (SEM) of five parallel samples from two independent experiments. *P ˂ 0.05, **P ˂ 0.01. b Neutral red-based cell viability analysis of miR negative control (miR-neg) and miR-342-3p (miR-342-3p) mimic-transfected RAW264.7 cells 48 h after miRNA mimic transfection. Each data point represents the mean and SEM of four independent experiments. *P ˂ 0.05. c Resorufin-based cell viability analysis of miR-negative control and miR-342-3p mimic-transfected RAW264.7 cells 48 h after miRNA mimic transfection. Each data point represents the mean and SEM of four independent experiments. *P ˂ 0.05. d Effect of miR-342-3p on cell cycle progression in RAW264.7 cells. Cell cycle distribution was evaluated by slide-based imaging cytometry after PI staining. Each data point represents the mean and standard deviation (SD) of three independent experiments. *P ˂ 0.05. e Representative dot plots of laser-scanning imaging cytometry analysis of AV- and PI-labeled miR negative control and miR-342-3p mimic-transfected RAW264.7 cells 48 h after miRNA mimic transfection. f Viable (PI-, AV-), early apoptotic (PI-, AV+) and late apoptotic (PI+, AV+) cell distribution 48 h following miR-negative control and miR-342-3p mimic transfection into RAW264.7 cells. Each data point represents the mean and SD of three independent experiments. *P ˂ 0.05