Fig. 2
From: De novo and rare mutations in the HSPA1L heat shock gene associated with inflammatory bowel disease
Effects of the HSPA1L variants on HSP70/HSP40-mediated refolding heat-denatured luciferase. a Reactivation of heat-denatured luciferase in the presence of each HSPA1L variant (4 μM). Luciferase activity in the presence of HSPA1L WT at 120 minutes was set as 100%. ** indicates P < 0.01 for the comparison between HSPA1L WT and each variant by Dunnett’s test (n = 3–6). b Dominant negative effects of Gly77Ser, Leu172del, and Ser277Leu in refolding activity of each HSPA1L variant (2 μM) in the presence of HSPA1L WT (2 μM). Refolding activity of HSPA1L WT (2 μM) only was set as 100%. ** indicates P < 0.01 for the comparison between HSPA1L WT only and each variant by Dunnett’s test (n = 3–6). c Dominant negative effects of Gly77Ser, Leu172del, and Ser277Leu in refolding activity of each HSPA1L variant (2 μM) in the presence of HSPA1A WT (2 μM). Refolding activity of HSPA1A WT (2 μM) only was set as 100%. * or ** indicates P < 0.05 or P < 0.01, respectively, for the comparison between HSPA1A WT only and each variant by Dunnett’s test (n = 3–4). The activity of the previously known mutation Lys73Ser was measured as a positive control for loss-of-function and dominant negative mutant. The bars represent the standard deviation. Data are representative of two independent experiments