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Fig. 3 | Genome Medicine

Fig. 3

From: Identification and characterization of a FOXA2-regulated transcriptional enhancer at a type 2 diabetes intronic locus that controls GCKR expression in liver cells

Fig. 3

Haplotype-specific H3K27Ac levels and FOXA2 binding by ChIP-qPCR. Chromatin was immunoprecipitated and H3K27Ac levels and FOXA2 binding was determined by qPCR using a custom TaqMan SNP Genotyping Assay for rs780094. The data represent the haplotype-specific enrichments over the input, normalized to a region of GRB10 with no H3K27Ac marks or TF binding. a Basal H3K27Ac enrichment (three experiments with three replicates each [n = 9]). b FOXA2 enrichment in FOXA2-transfected HepG2 cells (three experiments with two, two, and three technical replicates, respectively; n = 7). Insulin (100 nM) reduced the binding of FOXA2 to the CGG haplotype. In both panels, error bars represent standard deviation of all technical replicates. The asterisks depict statistical significance (**p ≤ 0.01; ***p ≤ 0.005; two-tailed t-test for comparison between the haplotypes; one-way ANOVA for comparisons between treatments (b))

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