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Fig. 5 | Genome Medicine

Fig. 5

From: Integrative analysis reveals functional and regulatory roles of H3K79me2 in mediating alternative splicing

Fig. 5

Functional validation of H3K79me2-mediated SE site switching in AML cell lines. a DOT1L knockdown by siRNA transfection in GM12878, MV-4-11, and MOLM14 cells. b DOT1L knockdown slowing cell proliferation for MV-4-11 and MOLM14 cells but not GM12878 cells. Cell proliferation was evaluated over 4 days of incubation in the CCK8 assay. c ChIP-qPCR detection showing reduced H3K79me2 enrichment levels for five specific SE sites after DOT1L knockdown. d Exon skipping site switching to the inclusion isoform after the decrease in H3K79me2 levels in five specific gene loci in AML cells

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Genome Medicine

ISSN: 1756-994X

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