Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA

Table 1 List of genes and regions covered in the panel

Gene	Target region	Hotspot position	No. of amplicons
AKT1	Hotspot	E17	4
		AA23–59
		AA65–94
BRAF	Hotspot	V600	1
Her2	Hotspot	S310	14
		AA428–438
		AA746–797
		AA832–986
HRAS	Hotspot	AA3–35 (G12 and G13)	3
HRAS	Hotspot	AA49–77 (Q61 and A66)	3
IDH2	Hotspot	AA 132–162	1
KRAS	Hotspot	G12	1
SF3B1	Hotspot	K700	1
ESR1	Part of exons	Exon 8–10 (LBD)	10
SMAD4	Part of exons	Exon 8–12	10
CDH1	All exons		46
CDKN1B	All exons		9
FOXA1	All exons		18
GATA3	All exons		23
MAP2K4	All exons		22
MAP3K1	All exons		75
PIK3CA	All exons		59
PIK3R1	All exons		11
PTEN	All exons		24
RUNX1	All exons		24
TP53	All exons		21

ISSN: 1756-994X