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Table 1 List of genes and regions covered in the panel

From: Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA

Gene Target region Hotspot position No. of amplicons
AKT1 Hotspot E17 4
AA23–59
AA65–94
BRAF Hotspot V600 1
Her2 Hotspot S310 14
AA428–438
AA746–797
AA832–986
HRAS Hotspot AA3–35 (G12 and G13) 3
AA49–77 (Q61 and A66)
IDH2 Hotspot AA 132–162 1
KRAS Hotspot G12 1
SF3B1 Hotspot K700 1
ESR1 Part of exons Exon 8–10 (LBD) 10
SMAD4 Part of exons Exon 8–12 10
CDH1 All exons   46
CDKN1B All exons   9
FOXA1 All exons   18
GATA3 All exons   23
MAP2K4 All exons   22
MAP3K1 All exons   75
PIK3CA All exons   59
PIK3R1 All exons   11
PTEN All exons   24
RUNX1 All exons   24
TP53 All exons   21