Fig. 1
From: CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling
CRISPR-SONIC enables homology-independent IRES-GFP integration in mouse cells. a Schematic showing the target genomic locus, guide RNAs, and donor plasmid. b Neuro2A cells were transfected with indicated plasmids. Five days post-transfection, cells were trypsinized, washed with PBS, re-suspended in PBS, and analyzed by flow cytometry for detection of GFP-positive cells. c Statistical analysis of percentage of GFP-positive cells. Reported as mean percentage. Error bars are s.d. (n = 3). d PCR detecting genomic integration and representative sanger sequencing. M, molecular weight DNA ladder