Fig. 5

DNA methylation changes in septic monocytes parallel organic damage. a DNA methylation heatmap of CpG changes in relation to the SOFA score (represented on the top of the heatmap). Spearman’s correlation was used with p < 0.01, r > 0.6. A scale is shown at the bottom, wherein beta values range from − 4 (lower DNA methylation levels, blue) to + 4 (higher methylation levels, red). b GO categories resulted from GREAT analysis of differentially methylated CpGs related to SOFA. c Bubble scatterplot of TF enrichment for hypermethylated and hypomethylated CpGs. The x-axis shows the percentage of windows containing the motif, and the y-axis shows the fold enrichment of the motif. Bubbles are colored according to TF family. p value is indicated by bubble size (selected TF with p ≤ 1e⁻⁰⁶ for hypermethylated and hypomethylation regions). d Box plots showing β-values obtained of genes significantly correlating with the SOFA score