Fig. 3

The abnormal activation of B cells in AML samples. a Normalized BCR CDR3 count in AML and non-tumor groups. The number of CDR3s was normalized by the number of total sequencing reads and one minus blast percentage (pathologically estimated tumor purity) in each sample. b BCR CPK in AML and non-tumor groups. c IgH SHM rate in AML and non-tumor groups. The p values in a, b, and c were calculated using the two-sided Wilcoxon rank-sum test. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. indicates not significant. d Distribution of 9 Ig isotypes across AML and non-tumor groups. e The regression curves of 8 Ig isotype fractions against age in AML samples. IgE was excluded due to the extremely low fraction in most samples. f Visualization of Ig isotype class switching in AML and non-tumor groups. Circle size represents the fraction of Ig isotypes, which is the number of IgH clusters carrying a given Ig isotype divided by the total number of IgH clusters in each group. Lines connecting two circles indicate co-existence of two isotypes in one cluster, with line width proportional to the number of such cluster divided by the total number of IgH clusters in each group