Fig. 4

Characteristics of artifactual variants identified by FIREVAT in publicly available VCF callsets. Analysis of artifactual variants identified by FIREVAT using MuTect2 callset of multiple TCGA cohorts and multi-region WES breast cancer samples (SRP070662). From the left, the first plot for each sample group shows the profiling of the sequencing artifactual signature occurrences and weights using unrefined mutations. The bar color intensity (white to red) represents the median weight of the observed artifactual signature and the bar length represents the number of samples that had the corresponding signature as the most heavily weighted artifactual signature. The artifactual signatures with a median weight higher than 0.2 are highlighted in bold font. The second plot shows the distribution of artifactual signatures among the original (green: Ori), refined (blue: Ref), and artifactual (orange: Art) sets of mutations, respectively. The last plot shows the enrichment of sequence motifs in the artifactual variants 10 bases upstream and downstream of each variant position