Fig. 1

Neoantigen detection in low mutation burden CRC. a Schematic overview of the experimental design. b Patient characteristics including HLA class I phenotypes and MMR status of the tumors. c Total number of transcribed, non-synonymous mutations per patient. d Heatmaps showing the relative expression for template genes (left) and gene set (right) used to determine the consensus molecular subtypes of CRC samples. Color saturation indicates the statistical significance; red and blue indicate the direction of change. The samples analyzed included the tumors that were investigated for neoantigen reactivity and additional 15 CRC samples for which RNA sequencing was available in-house.