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Table 2 List of FACS antibodies and reagents

From: Methylome-based cell-of-origin modeling (Methyl-COOM) identifies aberrant expression of immune regulatory molecules in CLL

Reagent

Clone

Supplier

Cat #

APC anti-human CD5

UCHT2

BioLegend

300612

eBioscienceâ„¢ CD152 (CTLA-4) PerCP-eFluor 710

14D3

Thermo Fisher Scientific

46-1529-42

eBioscienceâ„¢ CD276 (B7-H3) PE-Cyanine7

7-517

Thermo Fisher Scientific

25-2769-41

eBioscienceâ„¢ Fixable Viability Dye eFluorâ„¢ 506

 

Thermo Fisher Scientific

65-0866-14

eBioscienceâ„¢ IC Fixation Buffer

 

Thermo Fisher Scientific

00-8222-49

eBioscience™ Permeabilization Buffer (10×)

 

Thermo Fisher Scientific

00-8333-56

eBioscienceâ„¢ TIGIT PE-Cyanine7

MBSA43

Thermo Fisher Scientific

25-9500-42

Human TruStain FcXâ„¢ (Fc Receptor Blocking Solution)

 

BioLegend

422302

PE anti-human CD85k (ILT3, LILRB4) Antibody

ZM4.1

BioLegend

333007

PE/Dazzleâ„¢ 594 anti-human CD19 Antibody

HIB19

BioLegend

302252

PerCP/Cyanine5.5 anti-human CD2

RPA-2.10

BioLegend

300215