Table 3 Comparison of sequencing, PCR, and IHC for determination of tumor viral status
From: Clinical and molecular characterization of virus-positive and virus-negative Merkel cell carcinoma
ID | TMB | TMB category | PCR # primer sets | IHC | ViroPanel | Virus-positive Evidence | Add’l VP Evidence | UV | VP or VN |
|---|---|---|---|---|---|---|---|---|---|
MCC001 | 8.5 | Intermediate | 2 | 1 | Positive | ViroPanel | PCR IHC | 0 | VP |
MCC002 | 30.4 | High | 0 | 0 | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC003 | 0.0 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC004 | 6.6 | Intermediate | Â | Â | Â | Â | Â | 0 | VP |
MCC005 | 1.6 | Low | Â | 1 | Positive | ViroPanel | IHC | 0 | VP |
MCC006 | 3.7 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC007 | 9.6 | Intermediate | 1 | 0 | Negative | ViroPanel | Â | 0 | VN |
MCC008 | 2.7 | Low | 5 | 1 | Positive | ViroPanel | PCR IHC | 0 | VP |
MCC009 | 28.2 | High | Â | 0 | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC010 | 4.3 | Low | Â | 1 | Positive | Â | IHC | 0 | VP |
MCC011 | 11.7 | Intermediate | 0 | 1 | Negative | UV TM | Â | 1 | VN |
MCC012 | 0.0 | Low | Â | 0 | Â | Â | Â | 0 | VP |
MCC013 | 3.2 | Low | 5 | 1 | Positive | ViroPanel | PCR IHC | 0 | VP |
MCC014 | 3.7 | Low | Â | 1 | Positive | ViroPanel | Â | 0 | VP |
MCC015 | 27.7 | High | 0 | 0 | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC016 | 23.0 | High | Â | Â | Â | UV TM | Â | 1 | VN |
MCC017 | 2.5 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC018 | 1.6 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC019 | 4.3 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC020 | 27.7 | High | Â | 0 | Negative | UV TM | Â | 1 | VN |
MCC021 | 25.0 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC022 | 3.7 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC023 | 19.2 | Intermediate | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC024 | 3.2 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC025 | 0.0 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC026 | 4.3 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC027 | 2.7 | Low | 4 | 1 | Positive | ViroPanel | PCR IHC | 0 | VP |
MCC028 | 29.3 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC029 | 5.3 | Low | Â | 0 | Â | Â | Â | 0 | VP |
MCC030 | 38.9 | High | 0 | 0 | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC031 | 1.6 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC032 | 1.1 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC033 | 5.3 | Low | Â | 1 | Positive | ViroPanel | IHC | 0 | VP |
MCC034 | 28.8 | High | 0 | 0 | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC035 | 11.2 | Intermediate | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC036 | 3.2 | Low | 4 | 1 | Positive | ViroPanel | PCR IHC | 0 | VP |
MCC037 | 4.8 | Low | Â | 1 | Positive | ViroPanel | IHC | 0 | VP |
MCC038 | 1.6 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC039 | 22.4 | High | Â | Â | Â | UV TM | Â | 1 | VN |
MCC040 | 3.7 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC041 | 2.1 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC042 | 2.1 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC043 | 4.3 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC044 | 5.3 | Low | 2 | 1 | Positive | ViroPanel | PCR IHC | 0 | VP |
MCC045 | 2.1 | Low | Â | 1 | Positive | ViroPanel | IHC | 0 | VP |
MCC046 | 20.8 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC047 | 2.1 | Low | Â | 1 | Positive | ViroPanel | IHC | 0 | VP |
MCC048 | 26.6 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC049 | 34.1 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC050 | 4.8 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC051 | 3.2 | Low | Â | 1 | Positive | ViroPanel | IHC | 0 | VP |
MCC052 | 2.1 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC053 | 0.0 | Low | 3 | 0 | Â | Â | PCR | 0 | VP |
MCC054 | 3.7 | Low | 3 | 1 | Positive | ViroPanel | PCR IHC | 0 | VP |
MCC055 | 30.9 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC056 | 3.7 | Low | Â | Â | Positive | ViroPanel | Â | 0 | VP |
MCC057 | 29.8 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC058 | 1.1 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC059 | 19.2 | Intermediate | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC060 | 1.1 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC061 | 2.1 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC062 | 4.8 | Low | 4 | 0 | Positive | ViroPanel | PCR | 0 | VP |
MCC063 | 27.2 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC064 | 25.6 | High | Â | Â | Â | UV TM | Â | 1 | VN |
MCC065 | 13.3 | Intermediate | Â | Â | Â | UV TM | Â | 1 | VN |
MCC066 | 18.6 | Intermediate | Â | Â | Â | UV TM | Â | 1 | VN |
MCC067 | 0.5 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC068 | 2.1 | Low | Â | Â | Â | Â | Â | 0 | VP |
MCC069 | 4.3 | Low | Â | 1 | Positive | ViroPanel | IHC | 0 | VP |
MCC070 | 29.8 | High | Â | Â | Negative | UV ViroPanel TM | Â | 1 | VN |
MCC071 | 3.2 | Low | Â | Â | Â | Â | Â | 0 | VP |