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Fig. 4 | Genome Medicine

Fig. 4

From: Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis

Fig. 4

Surface expression of the co-stimulatory molecules CD80 and CD86 marks a subset of activated Tregs in vivo. a, b Expression of the B7 molecules CD80 (a) and CD86 (b) was assessed ex vivo by flow cytometry in CD4+ T cells from four healthy donors. Scatter plots depict the frequency (median) of CD80+ and CD86+ T cells within the CD45RA Teff (mTeffs; black) and CD45RA CD127lowCD25+ Treg (mTregs; blue) populations. c Scatter plot depicts the frequency (median) of FOXP3+ cells within total (black), CD80+ and CD86+ CD4+ T cells. d Gating strategy for the delineation of the Treg subsets according to the intracellular expression of the canonical Treg transcription factors FOXP3 and HELIOS. Scatter plot depicts the frequency of HELIOS+FOXP3+ (blue) and HELIOSFOXP3+ (red) subsets in each of the four donors within total mTregs, CD80+ mTregs and CD86+ mTregs. e Co-expression of CD86 and the CD4+ T cell activation markers CTLA-4 and HLA-DR was assessed by flow cytometry in CD86+ (red) and CD86 (blue) mTregs. f Scatter plots depict the frequency (median) of CTLA-4hi HLA-DR+ cells within CD86+ and CD86 mTregs. Expression of CTLA-4 was assessed by intracellular immunostaining

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