Fig. 3

Genomic co-localization of ASXL3 and BRD4 in SCLC. a Distribution of ASXL3 binding to genomic regions in the human SCLC cell line NCI-H1963, as assessed by chromatin immunoprecipitation sequencing (ChIP-seq) using two different ASXL3 specific antibodies. Peak annotation of ASXL3 is summarized in a pie chart format. b Sorted and centered heatmaps generated from ChIP-seq data analyses show the occupancy of ASXL3, BRD4, H3K27Ac, and H3K4me1 in NCI-H1963 SCLC cells. All rows are centered on ASXL3 peaks based on the ranking of signals. The published ATAC-seq data from the same cell line (GSM3321013) was also centered on ASXL3 peaks. c A Venn-diagram presentation of the overlap between ASXL3 and BRD4 peaks. d Representative tracks showing chromatin occupancy of H3K27Ac, BRD4, and ASXL3 binding sites. e The log2 fold-change heatmaps show the occupancy of H3K27Ac levels (left panel) and BRD4 levels (right panel) in cells transduced with ASXL3 shRNAs or non-targeting shRNA at TSS and non-TSS regions. f The average plot shows the global reduction of H3K27Ac (left panel) and BRD4 (right panel) in ASXL3-depleted cells. g The box plot shows the BRD4 occupancy in cells transduced with ASXL3 shRNAs or non-targeting shRNA at TSS and non-TSS regions. h Representative tracks showing chromatin occupancy of H3K27Ac and BRD4 at TCF4 (left) or SMARCA2 (right) gene loci