Fig. 1

An overview of the HiSIF algorithm. a Left: the schematic diagram of HiSIF. Right: the flowchart of the HiSIF algorithm including two modules: Quality Control and Classification of Hi-C interactions. b A distribution of USF counts frequency where a clear distinction of two Poisson means was observed in both MCF7 and MCF10a datasets. c Distribution of the genomic distance between every pair of ligated DNA segments where the result showed that the probability of background ligations follows a power law distribution. d The percentage of overlaps of SIFs between GM12878 replicate 1 and replicate 3–9 in various fragment sizes. e The percentage of overlaps of SIFs between GM12878 replicate 1 and replicate 3–9 in various FDR cutoffs