Fig. 3

Unsupervised clustering analysis of immune cells in PDAC primary tumors and metastatic lesions. Tumor-infiltrating lymphocytes (TILs) from primary tumors and metastatic lesions are mixed together (a) and form two main clusters (b). One of the clusters (c0) showed higher expression of genes associated with T cell exhaustion (c) and those cells also express a higher level of Ki67 gene (d). The tumor-associated macrophages (TAMs) from primary tumors and metastatic lesions form separate clusters (e). Heatmap shows distinct gene expression patterns between the two TAM populations (f) and the genes specifically express in the TAMs associated with the primary tumors are enriched in processes related to extracellular matrix (left panel in g) and wound healing (right panel in g). The expression level (Y-axis) in c and d is the logarithm-transformed ratio of the UMI counts of the gene(s) of interest over the total UMI counts in each individual cell. GO Gene Ontology