Fig. 1

OriPrint is able to stratify HGSOC on the basis of its cell of origin. a Schematic representation of the experimental pipeline. FI, fimbrial epithelium; OSE, ovarian surface epithelium; HGSOC high-grade serous ovarian cancer; FI-like, tumors originating from the fimbrial epithelium; OSE-like, tumors originating from the ovarian surface epithelium. b Top: PCA analysis of FI and OSE samples (n = 11 and 8, respectively) from the IEO cohort (purple and orange, respectively) considering ORIPrint CpGs. Bottom: Hierarchical clustering of the same samples, distance = Pearson’s correlation. c Top: PCA analysis of FI and OSE samples (tones of purple and orange, respectively) from IEO, Omaha (n = 5 and 5, respectively) and Melbourne (n = 6 FI) cohorts considering OriPrint CpGs in the space defined by normal samples. Bottom: Hierarchical clustering of the same samples, distance = Pearson’s correlation. d PCA analysis of normal and tumor samples (n = 24) from the IEO cohort, annotated using Pearson’s correlation-based classification in the space defined by normal samples. e PCA analysis of normal samples from IEO cohort and tumor samples from the Melbourne cohort (n = 85), annotated using Pearson’s correlation-based classification both in the space defined by OriPrint and normal samples (left) and by the whole set of CpGs (right)