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Fig. 5 | Genome Medicine

Fig. 5

From: Characterization of the dual functional effects of heat shock proteins (HSPs) in cancer hallmarks to aid development of HSP inhibitors

Fig. 5

Dual functional effects of individual HSPs in proliferation and metastasis. a HSPs with dual functional effects in proliferation and metastasis. Dots denote HSPs with dual functions in promoting both proliferation and metastasis (dark blue), inhibiting both proliferation and metastasis (cyan), promoting proliferation but inhibiting metastasis (red), inhibiting proliferation but promoting metastasis (magenta), respectively. Only instances have significant associations with both proliferation and EMT in a given cancer type were shown. Four arrows denote four directions, including proliferation (+) EMT (+), proliferation (+) EMT (−), proliferation (−) EMT (+), and proliferation (−) EMT (−), respectively. b Correlation and enrichment of DNAJC9 and HSPA14 with proliferation and EMT. c Reverse-transcription polymerase chain reaction was performed to confirm siRNA knockdown of HSPA14 or siDNAJC9. d Cell proliferation. Growth rates of A549 cells using WST-1 reagent. Absorbance was measured at λ = 450. e Western analysis of E-cadherin and vimentin expression. GAPDH was used as loading control. f Representative images of A549 cells transfected with siRNA for HSPA14 or DNAJC9 for 48 h and immunostained with E-cadherin (red) or vimentin (green). Scale bars, 10 μm. The plots at the right show the quantification of the intensity of E-cadherin (siCON, n = 65 (cells); siHSPA14, n = 80; siDNAJC9, n = 64) or vimentin (siCON, n = 64; siHSPA14, n = 64; siDNAJC9, n = 64). Error bars denote the standard deviation

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