Fig. 2
Synteny plot of hybrid resistance/virulence plasmid 1 (pPBIO1953_NDM-1) and resistance plasmid 2 (pPBIO1953_CTX-M-15). The plot depicts pairwise BlastN comparisons (E value 1e−10) between pPBIO1953 plasmid 1 and 2 and pK2044 and pKCTC2242. Alignment lengths were required to be at least 1% of the smaller replicon in the comparison to be included. Direct comparisons are colored with red hues whereas reverse comparisons are colored with blue hues. Boxes on top depict CDS on the forward strand, and those at the bottom depict CDS on the reverse strand. Boxes with a dotted outline are annotated as pseudo CDS. Plasmid 1 of PBIO1953 combines virulence features, such as aerobactin, with acquired antimicrobial resistance genes (e.g., blaNDM-1), which are missing from typical virulence plasmids of hypervirulent strains NTUH-K2044 and KCTC 2242. Note that PBIO1953 plasmid 2 carries several metal resistance genes (pco and sil genes) present on hvKP virulence plasmids but absent from plasmid 1