Fig. 5
From: 5′-tRF-GlyGCC: a tRNA-derived small RNA as a novel biomarker for colorectal cancer diagnosis
Co-culture with CRC cells increased 5′-tRF-GlyGCC of blood cells via ALKBH3. a, b The levels of 5′-tRF-GlyGCC in PENG-EBV (a) or THP-1 (b) cells co-cultured with or without CRC cells was tested by RT-PCR. c The levels of ALKBH3 mRNA in PENG-EBV cells co-cultured with or without CRC cells were tested by RT-PCR. d The protein levels of ALKBH3 in PENG-EBV cells co-cultured with or without CRC cells were checked by western blot analysis and quantitatively analyzed. e, f PENG-EBV cells pre-transfected with sh-control or sh-ALKBH3 were further co-cultured with or without CRC cells for 48 h; the expression of ALKBH3 e and 5′-tRF-GlyGCC f in PENG-EBV cells were checked. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, ** p < 0.01 compared with control; NS, no significant