Fig. 6

PRMT1 deficiency protects Apc^min/+ mice against DSS-induced CRC progression. a Schematic diagram of DSS-induced CRC in C57BL/6 J-Apc^min/+ mice with high-fat diet and related treatments. Tissue collection, analysis, and survival end-point analyses were performed at day 150 after the first DSS treatment. b Immunoblot analyses of PRMT1 and H4R3me2a in colon tissues (left) and hematoxylin and eosin (H&E) staining of colon tumors (right) from Apc^Min/+-Ctrl and Apc^Min/+-PRMT1^KD mice after the indicated LV infection. c Numbers and size of colon tumors found in Apc^Min/+-PRMT1^KD mice (n = 12) compared with Apc^Min/+-Ctrl mice (n = 12). Results are shown as mean ± s.d.; **P < 0.01 compared with the control mice. d Survival curves of Apc^Min/+-Ctrl (n = 12) and Apc^Min/+-PRMT1^KD (n = 12) mice. Statistical significance was determined by Kaplan–Meier log-rank test; *P < 0.05. e, f Quantitative real-time PCR analysis of indicated mRNAs normalized to GAPDH (e) and western blot analysis of indicated proteins normalized to histone H4 and Hsp70 (f) from colon tissues from Apc^Min/+-Ctrl and Apc^Min/+-PRMT1^KD mice. Results are shown as mean ± s.d. from 12 mice each; **P < 0.01 compared with the control mice. g Immunoblot analyses of PRMT1 and H4R3me2a in colon tissues (left) and hematoxylin and eosin (H&E) staining of colon tumors (right) from Apc^Min/+-PBS and Apc^Min/+-AMI-1 mice. h Numbers and size of colon tumors found in Apc^Min/+-AMI-1 mice (n = 12) compared with Apc^Min/+-PBS mice (n = 12). Results are shown as mean ± s.d.; *P < 0.05 compared with the control mice. i Survival curves of Apc^Min/+-PBS (n = 12) and Apc^Min/+-AMI-1 (n = 12) mice. Statistical significance was determined by the Kaplan–Meier log-rank test. *P < 0.05. j, k Quantitative real-time PCR analysis of indicated mRNAs normalized to GAPDH (j) and western blot analysis of indicated proteins normalized to histone H4 and Hsp70 (k) from colon tissues from Apc^Min/+-PBS and Apc^Min/+-AMI-1 mice. Results are shown as mean ± s.d. from 12 mice each; *P < 0.05, **P < 0.01 compared with the control mice. l Representative IHC staining of PRMT1, H4R3me2a, TNS4, EGFR, and Ki67 in colon tumor tissues of C57BL/6 J-Apc^Min/+ mice from indicated groups