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Fig. 4 | Genome Medicine

Fig. 4

From: Deletion of a non-canonical regulatory sequence causes loss of Scn1a expression and epileptic phenotypes in mice

Fig. 4

Increased seizure susceptibility and abnormal EEG in heterozygous 1b deletion mice. ad Latency measures were observed after an i.p. injection of 80 mg/kg PTZ over the course of a 30-min trial. Reduced latencies to first jerk, loss of righting, generalized clonic seizure, and full tonic extension were observed in 1b+/− mice when compared to WT littermate controls. EEG was collected using a wireless telemetry system before and after an i.p. injection of 80 mg/kg PTZ. e, f Representative EEG traces of WT and 1b+/− mice during baseline EEG recording and subsequent PTZ response. Powerband calculations and spiking events were automatically scored. g 1b+/− mice had significantly more spiking events and spike trains during baseline EEG acquisition when compared to WT. Scored spiking events are shown on a 1b+/− representative trace and indicated by red lines. h 1b+/− mice also had significantly higher power across all frequency bins, Delta (0–4 Hz), Theta (4–8 Hz), Alpha (8–12 Hz), Sigma (12–16 Hz), and Beta (16–30) during baseline when compared to controls. Finally, seizure susceptibility was confirmed with EEG after PTZ administration. i, j Reduced latencies to seizure onset and death were observed in 1b+/−mice. *, p < 0.05, t test

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