Fig. 1
From: Deconvolution of cell type-specific drug responses in human tumor tissue with single-cell RNA-seq
a Schematic illustration of experimental and analytical methods for slice culture drug perturbation and scRNA-seq. b UMAP embedding of scRNA-seq profiles from acutely isolated biopsies and slice cultures from different regions of the same tumor (PW032) colored by sample origin. c Same as b but colored by the log-ratio of Chr. 7 to Chr. 10 average expression where a high ratio (red) indicates malignant transformation. d Same as b but colored by cell type. e Heatmap of average expression of marker genes from cell types in the tumor microenvironment in each cell type and sample from PW032. f Fractional abundance of each major cell type in each biopsy and slice culture sample from PW032. g Two-dimensional model projecting each transformed cell from PW032 biopsies and slice into four major GBM transformed populations colored by sample origin