Fig. 4

Tumor subclonal dynamics vary across patients. Models of clonal and subclonal populations which make up the cancers of metastatic patients, derived using PyClone [34]. Variant inputs include union of filter-passing alterations from each sampled time point delivered by the commercially available liquid-biopsy targeted panel-sequencing pipeline at the Broad Institute. Copy number information and purity were derived from ichorCNA. a, b Clonal prevalence dynamics, clustering, and inferred phylogenetic tree structure for patient RP-466, revealing generally unchanging populations in the tumor, with important drivers occupying early positions in cell lineages. c, d RP-527 clonal dynamics profile and inferred tree structure showing statistically significant clonal expansion of cell lineage marked by non-synonymous DDR2 and RNF43 variants. e, f RP-557 profile and tree showing the opposite trend as RP-527, with a decreasing cell population marked by RB1 mutation