Fig. 4

dPCR-based quantification of SVs in blood. a Schematic overview of quantification of tumor-specific SVs, identified by SHARC, in cfDNA from blood by using qPCR and dPCR. b Primer and probe design for dPCR. The wild-type upstream and wild-type downstream alleles share each one primer with the mutant allele. Three probes with different fluorescents were designed to specifically detect the mutant allele or one of the wild-type alleles. c Detection of two tumor-specific SVs in cfDNA from blood from four patients with prostate cancer at baseline and at the progression of disease with dPCR. Shown are VAF and d mutant molecules per milliliter plasma. e Quantification of SVs in longitudinal cfDNA samples from blood of patient Pros1. The graph depicts VAFs of SVs, treatment, laboratory parameters (prostate-specific membrane antigen (PSA), alkaline phosphatase (ALP)), and clinical progression of disease (PD)