Fig. 8

DHX30 deficiency in zebrafish cells leads to reduced formation of stress granules. a Representative confocal images of TIAL-1-labeled stress granules (green) in dhx30 wild-type (+/+) and homozygous mutants (−/−). Zebrafish underwent normal conditions or heat shock treatment at 42 °C. Nuclei were counterstained with DAPI (blue). b Analyses of TIAL-1-labeled stress granules per 50 nuclei. The total number of embryos assessed are 8, 9, 8, and 8 for dhx30 +/+ (28 °C), dhx30 −/− (28 °C), dhx30 +/+ (42 °C), and dhx30 −/− (42 °C), respectively. Data are presented as means ± standard error of mean based on the indicated number of embryos. ***: significantly different from DHX30+/+ (***p< 0.001; unpaired Student’s t test)