Fig. 7

AGR-H19-Rgof alleviates HFD-induced obesity. a Left: Representative pictures of C57BL/6 J mice on a HFD followed by indicated treatment. Right: Comparison of body weights of mice with indicated treatment. Mean±SD, n=10 animals per group, one-way ANOVA. b Gained body weight after 14 weeks on HFD followed by indicated treatment. Mean ± SD, n = 10 animals per group, one-way ANOVA. c Top: Representative images showing individual muscle pieces. Bottom: Normalized weights of individual muscles. Mean ± SD, n = 10, 9 animals per group, Student’s t test. d Weight measurements of fat tissues. Mean±SD, n = 10, 9 animals per group, Student’s t test. e Forelimb grip strength test of C57BL/6 J mice on a HFD followed by indicated treatment. Mean ± SD, n = 5 animals per group, Student’s t test. f, g Total running time (f) or speed (g) of C57BL/6 J mice on a HFD followed by indicated treatment. Mean±SD, n = 5 animals per group, Student’s t test. h H&E, immunofluorescence staining, and Oil Red O staining of TA, livers, and WAT of C57BL/6 J mice on a HFD followed by indicated treatment. Scale bars, 100 μm. i, j Statistical analysis of staining intensities of DMD (i) or Ub-DMD (j). Mean ± SD, n = 8 animals per group, Student’s t test. k Mean TA fiber area of C57BL/6 J mice on a HFD followed by indicated treatment. Mean±SD, n = 10, 9 animals per group, Student’s t test. l Representative images (top) and weight measurement (bottom) of livers. Mean ± SD, n = 10, 9 animals per group, Student’s t test. m Energy expenditure measurements of C57BL/6 J mice on a HFD followed by indicated treatment. Mean±SD, n = 4 animals per group, two-way ANOVA. n–p Serum insulin concentration (n), total cholesterol (o), or leptin concentration (p) of C57BL/6 J mice on a HFD followed by indicated treatment. Mean ± SD, n = 5, 5 (n), 10, 9 (o), or 7, 7 (p) animals per group, Student’s t test. No significance [n.s.], p > 0.05, *, p < 0.05, **, p < 0.01, ***, p < 0.001, ***, p < 0.0001