Fig. 4

Redistribution of repressive histone marks and combinatorial entropy changes in PC-PMDs (a) Average methylation levels inside PC-PMDs according to average H3K9me3 intensity (data from the BLUEPRINT project: ERX1199099 and ERX712768). Each point represents a PC-PMD, and points are colored according to the size of the PC-PMD. (b) Example of a long unmethylated PC-PMD (pink area) associated with an increase in H3K9me3 in patient MM15548. (c) Average methylation levels in PC-PMDs according to their average H3K27me3 intensity (data from the BLUEPRINT project: ERX1199099 and ERX712769). (d) Example of PC-PMDs (pink areas) with methylation loss at diagnosis associated with an increase in H3K27me3 marks. (e) Scatterplot of hypomethylated eloci as a function of their epipolymorphism in NPC and diagnosis samples (M#09). The color gradient corresponds to the point density (low is green; high is red). Two eloci populations can be distinguished. The first population (top box) had a moderate decrease in methylation associated with a strong epipolymorphism, and the second population (bottom box) had a low heterogeneity and a drastic methylation decrease at diagnosis. (f) DNA methylation levels by patient of eloci with partial demethylation (eloci with an epipolymorphism value between 0.4 and 0.8 in NPCs and remain at the same level in MM) per patient are shown at the top, and DNA methylation levels of eloci with a decreased epipolymorphism value at diagnosis (eloci with an epipolymorphism value between 0.4 and 0.8 in NPCs, and an epipolymorphism value <0.2 at diagnosis) per patient are shown at the bottom. Green segments show the average methylation level of NPCs for these loci. The circles under the patient labels represent the size of the population compared to all hypomethylated eloci. Patient M#19, as an example in Fig. 5e, is outlined in orange