The expressed mutational landscape of microsatellite stable colorectal cancers

Table 1 Clinicopathological characteristics and expressed TMB of the in-house series of primary MSS CRCs

	Patients with MSS CRC (n = 121)	TMB^a		Expressed TMB^a
	Patients with MSS CRC (n = 121)	Mean [95% CI]	p	Mean [95% CI]	p
All tumors	121 (100%)	161 [147–174]	–	38 [35–42]	–
Gender
Male	63 (52.1%)	156 [139–173]	0.44	37 [33–42]	0.49
Female	58 (47.9%)	166 [145–188]	0.44	40 [34–46]	0.49
Age, median [10–90th]	72.5 [54.7–85.4]
Above median age		158 [141–175]	0.71	38 [34–43]	0.99
Below median age		163 [142–185]	0.71	39 [34–44]	0.99
Tumor localization^b
Right	51 (42.1%)	177 [155–198]	0.088^c	44 [38–50]	0.037^c
Left	42 (34.7%)	155 [134–175]		36 [31–40]
Rectum	27 (22.3%)	142 [110–175]		33 [26–40]
Synchronous	1 (0.8%)	–		–
Cancer stage
I	1 (0.8%)	–	–	–	–
II	59 (48.8%)	166 [148–183]	Reference	39 [34–45]	Reference
III	49 (40.5%)	170 [145–195]	0.50^d	39 [34–45]	0.89^d
IV	12 (9.9%)	107 [87–127]	0.0003^d	32 [23–40]	0.099^d
Treatment prior to tumor sampling
Yes^e	4 (3.3%)	61 [− 9–131]	0.014	12 [–7–31]	0.016
No	117 (96.7%)	164 [151–178]	0.014	39 [36–43]	0.016
Adjuvant chemotherapy (non-available: n = 1)
Yes	36 (30%)	158 [128–189]	0.2	37 [30–43]	0.2
No	84 (70%)	162 [147–177]	0.2	39 [35–43]	0.2

^aNon-synonymous SNVs, frameshift indels, splice site mutations (for 3 of the tumors: mean of multiregional samples)
^bTumors in the transverse colon (n = 8) were considered right-sided
^cRight versus left and rectum, based on the Mann-Whitney U-test, excluding four rectal tumors treated with pre-operative radiotherapy (including the synchronous)
^dMann-Whitney U-test with stage II as a reference category
^ePre-operative radiotherapy for locally advanced rectal cancer

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