Table 1 Evaluation of the species identification of 50 Klebsiella spp. isolates by MALDI-TOF mass spectra using ribosomal marker mass profiles. Species identification of 800 MALDI-TOF mass spectra using 110 marker mass profiles was compared to the species identification assigned using WGS data. Specificity and sensitivity were computed on two levels: (i) whether the assignment to K. pneumoniae group or K. oxytoca group was correct (= “Group level”) and (ii) whether the correct species within each group could be assigned (= “Species level”)
Species identification by WGS | Identification of MALDI-TOF mass spectra using Marker Mass profiles based on 25 pre-defined ribosomal subunit proteins | |||
|---|---|---|---|---|
Group level | Species level | |||
Sensitivity [%] | Specificity [%] | Sensitivity [%] | Specificity [%] | |
K. pneumoniae | 98.8 | 100 | 70.0 | 100 |
K. variicola | 98.1 | 100 | 93.1 | 100 |
K. oxytoca | 99.2 | 100 | 80.5 | 99.4 |
K. michiganensis | 100 | 100 | 44.3 | 100 |
K. grimontii | 99.4 | 100 | 70.6 | 100 |