Fig. 2

Identification of host factors that promote HCoV infection of Vero E6 cells. Vero E6 cells transduced with a C. sabaeus-specific sgRNA library were infected with SARS-CoV-2 or OC43 at MOI 0.01, resistant cells reinfected at MOI 0.01, and sgRNAs in resistant clones sequenced. MAGeCK analysis of multiple replicates compared to uninfected control library replicates yielded log₂fold changes (log₂FC) that were plotted on the x-axis. Negative log₁₀-transformed false discovery rates (FDR) were plotted on the y-axis. Data are presented for SARS-CoV-2 (A) and OC43 (B) Vero E6 infections. The heat maps display the log₂FC for the 20 top-scoring genes comparing results for SARS-CoV-2 and OC43 infections. Genes targeted by significantly enriched sgRNAs (FDR < 0.25) were segregated into functional categories using PANTHER for SARS-CoV-2 (C) and OC43 (D) infections