Fig. 3

Identification of host factors that promote SARS-CoV-2 infection of HEK293T-hACE2 cells. HEK293T-hACE2 cells transduced with the Brunello sgRNA library were infected with SARS-CoV-2 at MOI 0.01 and sgRNAs in resistant clones sequenced. Resistant clones were reinfected with SARS-CoV-2 at MOI 0.01 or MOI 0.1 and sgRNAs in resistant clones sequenced. For all three infections, MAGeCK analysis of multiple replicates compared to uninfected control library replicates yielded log₂fold changes (log₂FC) that were plotted on the x-axis. Negative log₁₀-transformed FDR were plotted on the y-axis. Data are presented for the initial infection (A), MOI 0.01 reinfection (B), and MOI 0.1 reinfection (C). D The heat map displays the log₂FC for top-scoring genes (FDR < 0.1) across the three infections. The entire experiment was repeated at MOI 0.3, with sgRNAs sequenced from resistant clones in the initial infection (E) and reinfection (F). G The heat map displays the log₂FC for top-scoring genes (FDR < 0.25) across the two infections