Fig. 4

Identification of host factors that promote OC43 infection of HEK293T-hACE2 cells. HEK293T-hACE2 cells transduced with the Brunello sgRNA library were infected with OC43 at MOI 0.01 and sgRNAs in resistant clones sequenced. Resistant clones were reinfected with OC43 at MOI 0.01 or MOI 0.1 and sgRNAs in resistant clones sequenced. For all three infections, MAGeCK analysis of multiple replicates compared to uninfected control library replicates yielded log₂fold changes (log₂FC) that were plotted on the x-axis. Negative log₁₀-transformed FDR were plotted on the y-axis. Data are presented for the initial infection (A), MOI 0.01 reinfection (B), and MOI 0.1 reinfection (C). D The heat map displays the log₂FC for top-scoring genes (FDR < 0.25) across the three infections