Fig. 3
From: Single-cell genomic and transcriptomic landscapes of primary and metastatic colorectal cancer tumors
Transcriptomic differences between adjacent normal tissues and tumor tissues. A Heatmap showing the differentially expressed genes (DEGs) between adjacent normal and tumor tissues in each patient. Colors from blue to orange represent the expression level from low to high. The enriched gene ontology (GO) terms and p-value were showed in the right side of the heatmap. The full list of DEGs is summarized in supplementary table 2. B Immunofluorescence staining of CEACAM6 and LY6E in adjacent normal tissue and primary tumor tissue for another two patients. Both CEACAM6 and LY6E are highly expressed by tumor epithelial cells. Scale bar, 100 μm. C Bright-field images of patient-derived normal and tumor organoids (left panel). Representative bright-field images of PPAR inhibitor (FH535)-treated and untreated organoids (right panel). D Cell viability assay of PPAR signaling pathway inhibitor (FH535)-treated and untreated organoids. ** represents p-value < 0.05. E Cell apoptosis assay of PPAR inhibitor (FH535)-treated and control organoids. The results of fluorescence-activated cell sorting (FACS) are shown. Q2 represents late apoptotic cells, Q3 represents early apoptotic cells, and Q4 represents living cells. F Bar plot shows the ratio of apoptotic cells, light gray represents early apoptotic cells, and dark gray represents late apoptotic cells. P-values are calculated by t-test. ** represents p-value < 0.01. G Dot plot showing the proliferation ratio of cells. Different colors represent different drug treatment. 5-FU: 30 μM; GW9662: 30 μM; FH535: 15 μM. P-values are calculated by t-test. ** represents p-value < 0.01. NS represents not significant. There are 3 replications for each treatment. H The line plot showing the cell viability of tumor organoid (O#L) under different concentrations of drugs. The x-axis represents the concentration of PPAR inhibitor GW9662, and the y-axis represents the cell viability of tumor organoid with 5 days drug treatment. Colors from blue to red represent the concentration of WNT inhibitor XAV939 from low to high