Fig. 2

Proportional subtype assignment (PSA) of RCC and RCC cell lines. A, B Principal component analysis of the TCGA RCC cohort (C3) using expression data of the 174 signature genes. A TCGA cohorts of ccRCC (KIRC, n = 512), pRCC (KIRP, n = 287), and chRCC (KICH, n = 65) are displayed. B PSA were determined for tumors of C3 by computational deconvolution. A total of 246 RCC samples with maximum PSA values below 95% were considered as potential heterogeneous tumors (enlarged symbols). Their molecular subtype composition based on PSA is visualized by pie charts. Nineteen samples with \({P}_{psa}>0.05\) as determined by a permutation P-value approach are displayed by shaded pie charts with gray borders. C PSA for 14 RCC-derived cell lines were calculated using transcriptomic data as provided by the Broad-Novartis Cancer Cell Line Encyclopedia (CCLE) [37, 38] as well as the COSMIC Cell Lines Project (CCLP) [39, 40]. Asterisk indicates \({P}_{psa}<0.05\)