Fig. 3

Enrichment of 5ALA + gene signatures in myeloid-like aneuploid, diploid myeloid, and non-myeloid malignant cells. tSNE plot representation of the sing cells (N = 261,092) annotated by GBmap as malignant and myeloid cell types (A). The color code represents the proposed cell annotation from GBmap. Copy number variation (CNV) categories (aneuploid and diploid) based on were mapped as different colors (aneuploid: Orange and diploid: Green) (B). tSNE plot with Louvain clustering of myeloid cells from GBmap dataset (N = 134,405, as annotated by Gbmap) (C). tSNE representation of the myeloid-like aneuploid (N = 5171) (left) and diploid myeloid (N = 127,483) (right) cells. The color code represents the 5ALA + UCell enrichment score calculated based on the expression of the 5ALA + -specific genes (N = 251) (D). UCell scores of different gene signatures (5ALA + , GPM, Inf. wound, Inf. response, MES, and TNFα) across aneuploid myeloid (AM), diploid myeloid (DM), and non-myeloid-like malignant (malignant) cells (E). CIBERSORTx-derived signature matrix based on three cell annotations (DM, MLA, and Malignant) (F). Color code represents the z-scored expression. Selected differentially expressed genes are shown. Estimated fractions of different cellular states (MLA, DM, and Malignant) across unsorted Core and sorted 5ALA + and 5ALA − cells (G). Louvain clustering based on the single-cell RNA-seq data from GBmap (H). The color code represents 18 different clusters (cluster 0 to cluster 11). tSNE plots of GBmapdataset are shown where the color code represents the single-cell wise UCell scores for different gene signatures—MES1-like (I), MES2-like (J), Inf. wound response (K), GPM (L), MTC (M), and 5ALA + (N)