Fig. 5
Identification of rare infiltrative 5ALA + cell cluster within GBM invasive margin. GSEA score of 5ALA + gene signature (A), MES (B), and Inf. wound response (C) are shown for patient sample UKF#334, with enriched (red) and random (blue) spots. Spatial localization of the 5ALA + gene signature is marked (dashed box), where red (5ALA-INV) and green (5ALA-CT) boxes represent 5ALA + enriched spots. GSEA between 5ALA-CT and 5ALA-INV is shown as a bubble plot (D). Enrichment scores (− log10 FDR) are color-coded (yellow—high enrichment; black—low enrichment). H&E-stained tissue section of patient UKF#313 showing the necrotic core, pseudopalisading cells, and cellular tumor (CT) regions (E). Inferred CNV analysis of chromosomes 7 (F) and 10 (G) presented by a spatial surface plot where gain and loss of chromosomes 7 and 10 respectively are color-coded. GSEA of 5ALA + gene signature illustrated by a surface plot where GSEA score is depicted by color code and 5ALA + enriched spots marked (dashed box) (H). H&E-stained tissue section of patient UKF#269 showing CT and adjacent non-tumor (NT) regions (I). Inferred CNV analysis of chromosomes 7 (J) and 10 (K) is shown by a surface plot. GSEA of 5ALA + gene signature is shown by a surface plot where 5ALA + enriched spots are marked (dashed box) (M). H&E-stained tissue section of patient UKF#275 showing CT with surrounding pseudopalisading cells (M). The scaled spatial program score indicates the expression of the reactive immune program (N). GSEA score representing spatial arrangements of 5ALA + gene signature (O) and GPM (P) enriched spots (dashed box). Spatial locations of 5ALA + enriched spots are represented by the GSEA score where red indicates high enrichment of 5ALA + gene signature (Q). The 5ALA-INV and 5ALA-CT spots are indicated by red and green boxes, respectively. GSEA scores representing GPM (R), MTC (S), and hypoxia (T). Spatially weighted correlation analysis and spatial overlap of 5ALA + gene signature with established transcriptional signatures (U). Gene sets with high cross-correlation with 5ALA + gene signature are marked (dashed box) (U). CNV analysis of Chr7 (left) and Chr10 (right), across four samples (UKF#275, UKF#243, UKF#251, and UKF#334) (V). The color code indicates the gain or loss of chromosomes. Each box represents the average CNV value from 50 selected spots across 5ALA and NT regions. The stars indicate the significance of the p-value < 0.001