Fig. 4
From: Identification of novel genetic risk factors of dilated cardiomyopathy: from canine to human
Immunofluorescent staining of RNF207 in canine cardiac tissue. Healthy canine non-Dobermann cardiac tissue (top three rows) was stained for RNF207 to detect the differences in the expression or localization associated with disease and genotype. Additionally, tissue was stained for Hoechst in blue to show the nuclei and for actinin alpha 2 (ACTN2) in green to visualize the sarcomere structures. Localization is indicated from a transversal and longitudinal perspective. RNF207 is expressed in the cytoplasm of cardiomyocytes, intercalated disks (arrows), and perinuclearly (asterisks). In the cardiac tissue of the single DCM case homozygous for the RNF207c.1297-1G > A variant, a patchy expression resembling cellular mosaicism was detected (bottom row)