Fig. 1

Long-read ONT sequencing identifies novel transcripts in CRC. A Workflow for transcriptome reconstruction based on ONT sequencing data. The FLAIR- SQANTI3 pipeline was adopted to assemble transcripts from long-read ONT data based on GENCODE v34. B Transcript length distribution of different types. C Number of transcripts identified per gene by ONT sequencing. D Percentage of long-read ONT transcripts supported by Short-Read Illumina RNA-seq in 78 CRC and 10 adjacent normal samples. E Percentage of long-read ONT transcripts’ TSSs supported by CAGE peaks, or TTSs supported by 3’-seq peaks or presence of a poly(A) motif