Fig. 3

Identification of alternative splicing events in CRC. A A schematic diagram of the seven types of AS events including A5: Alternative 5’ Splice Site, A3: Alternative 3’ Splice Site, AF: Alternative First Exon, AL: Alternative Last Exon A3, MX: Mutually Exclusive Exon, RI: Retained Intron, and SE: Skipping Exon. B UpSet plot of interactions between the seven types of detected AS events in CRC. Percentage of seven types of AS events detected in CRC (upright). One gene may have up to all seven types of alternative splicing. C The volcano plot visualizing DEAS events between CRC and adjacent normal tissues. The red and blue points in the plot represent the DEAS events with statistical significance. D Enrichment analysis of genes involved DEAS events for hallmark pathways from MSigDB. Each row represents a hallmark geneset/pathway, whereas each column indicates a type of DEAS events. E Distribution of PSI for EIF4H, YWHAE, XPO1, and EEF1B2 in CRC and adjacent normal tissues. P values were calculated by two-sided Mann–Whitney test