Fig. 4

Identifications of prognostic DEAS events in CRC. A Top 30 prognostic DEAS events are shown, ranked by differential survival. AS events are labeled with gene name, AS event type, and the number of patients. Each AS event is depicted in the heatmap, including survival prognosis, differential status, and average PSI values. B Gene structure of the known and novel TSS of FHL2 transcript according to the ONT long-read transcriptome of CRC tissues. C Kaplan–Meier survival analysis of the correlation between three types of FHL2 DEAS events and patients’ overall survival rate, log-rank test. D Distribution of PSI for 3 types of FHL2 AF events in CRC and adjacent normal tissues, P values were calculated by Two-sided Mann–Whitney test