Fig. 7

SRSF1 sustains the exon 4–5 inclusion of TIMP1 in CRC. A RBPMap analysis shows the potential binding of several RNA-binding proteins in TIMP1 pre-mRNA. B RNA immunoprecipitation (RIP)-qPCR analysis of SRSF1 binding with TIMP1 pre-mRNA in SW480 cells. Data were shown as mean ± SD. P values were calculated by two-sided Student’s t test, *** P < 0.001. C Western blot and RT-PCR analysis of TIMP1 exon 4 and 5 skip after transfecting with si-SRSF1 or control siRNA in HCT-8 and SW480 cells. D Quantitative analysis of the western blot and RT-PCR results in C by the ImageJ software. Data were shown as mean ± SD. P values were calculated by two-sided Student’s t test, ** P < 0.01, *** P < 0.001. E MTT assay of HCT-8 and SW480 cells transfected with si-SRSF1, si-SRSF1 + si-TIMP1 Δ4-5, and control siRNA. Data were shown as mean ± SD. P values were calculated by two-sided Student’s t test, ** P < 0.01. F, G Tumor cell migration (F) and invasion (G) assay of HCT-8 and SW480 cells with indicated treatments. P values were calculated by two-sided Student’s t test, ** P < 0.01, *** P < 0.001. The migrated or invaded cells were quantified by counting in five fields. Scale bar, 100 μm