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Fig. 1 | Genome Medicine

Fig. 1

From: Small extrachromosomal circular DNA harboring targeted tumor suppressor gene mutations supports intratumor heterogeneity in mouse liver cancer induced by multiplexed CRISPR/Cas9

Fig. 1

Induction of primary mouse liver cancer by CRISPR/Cas9-mediated somatic mutagenesis in mice. A 34 TSGs and the Setd5 control site targeted by multiplexed CRISPR/Cas9. Each TSG is indicated with its related cancer signaling pathway. Hepatic delivery of SpCas9 and sgRNA expression plasmids is achieved by hydrodynamic tail vein injection (HTVI). B Effect of sgRNA dosage on CRISPR/Cas9-induced liver tumor formation. Each dot indicates one mouse. C Mouse liver specimen with or without tumor nodules (left) and H&E staining of a tumor nodule (right). D Microscopic IHC images of CRISPR/Cas9-induced liver tumors. T#1, T#2, and T#3 represent tumor nodules from three mice. AFP and GP73: HCC biomarkers; CK19: ICC biomarker. E Histology and IHC staining of a liver tumor nodule indicating three heterogeneous regions. Left: these three regions indicated with R1, R2, and R3 in H&E staining; Right: microscopic IHC images for these three regions

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Genome Medicine

ISSN: 1756-994X

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