Fig. 2

Target TSG mutation profiles in CRISPR/Cas9-induced mouse liver tumor nodules and single-cell clones. A Representative target mutation types of p53, Atm and Rb1 in a tumor nodule induced by SpCas9 together with 35 sgRNAs each at 0.8 mg and the frequencies of these mutations. sgRNA target sites with PAM and the cleavage position are indicated. Dash lines and nucleotides in curly bracket in the sequences denote deletion and inserted nucleotides, respectively. WT, wild-type; Del, deletion; Ins, insertion. The numbers flanking Del or Ins indicate the numbers of deleted or inserted nucleotides. B Representative target mutations of 21 TSGs with cumulative frequencies in the tumor nodule in A. Fourteen unedited gene are also listed on the right. C Mutational landscape of 35 targets in 22 primary tumor nodules, 4 parts from a single tumor nodule, and 37 single-cell clones as indicated. Top and right bars indicate the mean of the mutation frequencies for each sample and each gene, respectively. Cumulative matrix mutation frequencies are highlighted from the highest 8 in red to the lowest 0 in blue