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Fig. 3 | Genome Medicine

Fig. 3

From: Small extrachromosomal circular DNA harboring targeted tumor suppressor gene mutations supports intratumor heterogeneity in mouse liver cancer induced by multiplexed CRISPR/Cas9

Fig. 3

Alteration of target site mutations between subclones of single-cell clone 1C3 and 6C7 derived from mouse liver tumors. A Changes in target site mutation profiles between parental single-cell clones (i.e., 1C3 and 6C7) and their subclones (1C3-1, 1C3-2, 1C3-3, and 1C3-4 for 1C3 and 6C7-1, 6C7-2, 6C7-3, 6C7-4, 6C7-5, 6C7-6, 6C7-7, and 6C7-8 for 6C7). Gene targets and their mutation types are shown on top and at bottom, respectively. The numbers flanking Del or Ins indicated at bottom are the numbers of deleted or inserted nucleotides. √Frequency representing square root of mutation frequencies is indicated from the highest 1 in dark green to the lowest 0 in white. The top line chart and the boxplot under show the number of single-cell clones with a specific mutation type, i.e., number of clones, and the percentage distribution of a specific mutation type in all single-cell clones, i.e., proportion (%), respectively. B–E Representative target mutation oscillation of Rb1 (B), Lkb1 (C), Arid1a (D), and Smad4 (E) between parental clone 1C3 and its subclones

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Genome Medicine

ISSN: 1756-994X

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