Fig. 5

Oscillation of target site mutations during subcutaneous tumor cell grafts of 1C3-1. A Schematic of experimental outline. After subcutaneous implantation of the single-cell subclone 1C3-1 into SCID mice, tumors were formed and four tumor tissues (SG1, SG2, SG4, and SG5) from different mice collected at 2 weeks post inoculation. Two single-cell clones were derived from each of these four tumor tissues as indicated. Target site mutations were determined by targeted PCR amplicon deep sequencing of gDNA extracted from cells. SG, subcutaneous graft. B Changes in target site mutation profiles of single-cell clones from subcutaneous tumor cell grafts as indicated. Gene targets and their mutation types are shown on top and at bottom, respectively. The numbers flanking Del or Ins indicated at bottom are the numbers of deleted or inserted nucleotides. √Frequency representing square root of mutation frequencies is indicated from the highest 1 in dark green to the lowest 0 in white. The top line chart and the boxplot under show the number of single-cell clones with a specific mutation type, i.e., number of clones, and the percentage distribution of a specific mutation type in all single-cell clones, i.e., proportion (%), respectively. C–F Representative target mutation oscillation of Rb1 (C), Lkb1 (D), Col1a1 (E), and Rasa1 (F) across single-cell clones derived from subcutaneous tumor cell grafts