Fig. 6

Genomic instability in single-cell clones derived from CRISPR/Cas9-induced mouse liver tumors. A Microscopic images of spontaneous γH2AX and 53BP1 foci in primary mouse liver cancer cells. B Percentage of cells with > 5 γH2AX foci or 53BP1 foci. Columns indicate the mean ± S.E.M from three independent experiments with statistical significance detected by One-way ANOVA with Dunnett’s multiple comparison test (vs. NIH-3T3). *P < 0.05 and ***P < 0.001. C Microscopic images of micronuclei (MN) in primary mouse liver cancer cells. D Percentage of cells with micronuclei. Columns indicate the mean ± S.E.M from three independent experiments with statistical significance detected by One-way ANOVA with Dunnett’s multiple comparison test (vs. NIH-3T3). **P < 0.01 and ***P < 0.001. E Representative images of chromosomal aberrations in metaphase spread of primary mouse liver cancer cell lines. Red arrows and black arrows indicate chromosomal fragments and biarmed chromosomes, respectively. F Summary of metaphase spread analysis. In each indicated cell line, the number of metaphases, number of chromosomes per metaphase, and percentages of metaphases with biarmed chromosomes and with chromosomal fragments are calculated and shown