Fig. 8

Target site mutations of TSGs detected on eccDNA. A Circular barplot for target site mutations of 35 targets in gDNA and eccDNA in representative single-cell clone 1C3-1. The column in the inner circle for each target gene were relative read ratios of eccDNA to gDNA for each target site from deep sequencing data. The stacked barplot in the outer circle represents the mutation pattern at indicated target sites in eccDNA and gDNA. Mutation types are shown in color. Total read counts of eccDNA and gDNA are listed. *, invalid read counts at indicated sites, which are defined as < 0.1% of total read counts from eccDNA or gDNA. B, C Target site mutation distribution of representative TSGs Atm (B) and Rb1 (C) in gDNA and eccDNA. D–F Circularization junctions of eccDNA containing target sites for Atm (D), Rb1 (E), and Kmt2c (F). Circularization junction sequences as shown are determined by PCR with a pair of indicated outward primers followed by Sanger sequencing. Target sites with PAM and mutations are also indicated